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Feedback Tracking-FCS of Fluorescent Nanoparticles and Biomolecules
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The best way to study dynamic fluctuations in single§molecules or nanoparticles is to look at only one§particle at a time, and to look for as long as§possible. Brownian motion makes this difficult, as§molecules move along random trajectories that carry§them out of any fixed field of view. §§This thesis describes an instrument that tracks the§Brownian motion of single fluorescent molecules in§three dimensions and in real-time while measuring§fluorescence with nanosecond time resolution and§single-photon sensitivity. The apparatus increases§observation times by approximately three orders of§magnitude while improving data-collecting efficiency§by locking tracked objects to a high-intensity region§of the excitation laser.§§Applications of the instrument to the study of§quantum dot photon statistics and of the thermal§intramolecular motion of flexible polymer chains are§presented.
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