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Fungi and Cholesterol Oxidase Enzyme
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In this book, the possibility of production of §cholesterol oxidase enzyme from fungal isolates were §studied. These fungi were isolated from raw butter §and food oils. The study focused on four main aims: §a)surveyed the mycobiota in 30 samples of each of §raw butter and food oils by using five types of §isolation media, b) 432 isolates belonging to 31 §species and 14 genera were screened for their §abilities to produce cholesterol oxidase enzyme §using two assay techniques, cholesterol clear zone §(CCZ) and spectrophotometrically, C)studying the §effects of some environmental and nutritional §factors on cholesterol oxidase production by §Aspergillus niger MF 75, Geotrichum candidum RM 22 §and Penicillium corylophilum AM 99 (from survey §results, these three fungi were the best for enzyme §production), and d)purification and characterization §of cholesterol oxidase enzyme produced by these §isolates.
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